Inserm biology technician position at the Université Côte d’Azur
We are seeking a talented and motivated candidate with a Bachelor Degree +2 in biotechnology (for instance graduated from a IUT) for a technician position to work in the teams of Dr. Matteo RAUZI and Dr. Arnaud HUBSTIENBERGER at the Institut de Biologie Valrose (University Côte d’Azur). This job offer can eventually result in a permanent position.
Tasks to be fulfilled:
- Learning and conducting genetic crosses for both the Drosphila and Elegans model systems.
- Learning and applying confocal microscopy imaging techniques on fixed and living
- Conducting molecular biology and biochemical
- Preparing solutions and media for the members of the
- Helping the members of the teams in their
- Taking care of the orders for consumables and small
- Taking care of the safety
- Fostering cohesiveness among the people in the lab.
- Mastery of the English language
- Maintaining a lab notebook
- Team spirit
Interdisciplinary Doctoral project
at the interface between engineering, biology, physics and informatics
at the University Côte d’Azur
Studying the mechanisms and mechanics driving epithelial tube formation
The formation of epithelial tubes is essential to build organs responsible to direct vital factors outside-in, inside-out or within a living animal (e.g., food and water in gut, air in lungs, saliva from salivary-tubes, blood in blood-vessels etc.). Therefore, tubing plays a critical role in multicellular life organized in stratified layers (i.e., where an inside and an outside is established). Understanding the mechanisms and mechanics responsible for tube formation is thus of great importance. Tube formation can result from tissue in-pocketing. The mechanisms responsible for in-pocketing are not well understood. To dissect and study this process, we use the sea urchin gastrula: a quite simple (i.e., powerful) model system ideal for in vivo mechanical studies. Our preliminary data highlight a possible combination of coordinated and radially planar cell polarized mechanisms that could be responsible for simultaneous uniaxial folding and extension of the vegetal plate. By implementing infra-red femtosecond ablation coupled to 4D multi-view light sheet microscopy, drug and RNAi perturbation, µ-aspiration and indentation to measure tissue mechanical properties, in toto 4D segmentation and mathematical modelling, this work will shine new light on the mechanisms and mechanics driving tissue in-pocketing for tube formation.
The project will be developped in the Rauzi lab that gathers people from different backgrounds (biology, informatics, physics, and engineering) to generate an interdisciplinary and synergistic group in an international environment.
We are seeking a motivated and talanted candidate to develop this PhD project.
Send by the 12th of May 2021 a CV, a motivation letter, master scores/ranking and reference letters to firstname.lastname@example.org
RAUZI LAB: http://ibv.unice.fr/research-team/rauzi/
A joint PhD position in Neuroscience is available at the University of Turin (UNITO) and the University Côte d’Azur (UCA) within the framework of the Vinci Program 2020 (Università Italo-Francese/Université Franco-Italienne).
We are seeking for a highly motivated candidate, strongly interested in Experimental Neuroscience and Molecular Neurobiology and dedicated to high quality research. The research project deals with the identification of molecular mechanisms controlling mitochondrial function in postnatal neurogenic niches and their implication in cognitive disorders.
The project will focus on the mitochondrial dysfunction caused by deficiency of the transcriptional regulator Nr2f1 (also known as COUP-TFI) and will address the downstream target genes crucial for mitochondrial function and investigate its outcome on neuronal plasticity and function. Patients with NR2F1 haploinsufficiency have mild to severe neurodevelopmental cognitive disorders, such as intellectual deficiency, epilepsy, learning and language impairments. The final goal is to unravel the cellular and molecular mechanisms by which Nr2f1 controls mitochondrial function in neurons and how this is correlated with proper cognitive behavior.
The project will combine multiple methodologies ranging from gold-standard neuroanatomical approaches to advanced techniques, such as tissue clearing, light-sheet microscopy and 3D whole-brain reconstruction, and two-photon functional imaging, as well as genome-wide and in silico analyses and animal behavior.
The successful candidate will enroll as a PhD student in Neuroscience at the University of Turin under the co- direction of Prof. Silvia De Marchis and Dr. Michèle Studer. The candidate needs to have good communication skills in English and willing to work in Italy and France since the project will be carried out in the “Adult Neurogenesis” group at the Neuroscience Institute Cavalieri Ottolenghi at UNITO and in the “Development and Function of Brain Circuits” group at UCA.
The call will open on May 20, 2021 on the UNITO website (deadline for mid-June, 2021 – check the exact deadline on the platform) and the starting date of the PhD program is November 1st, 2021. The position is fully financed for four years.
If interested, please contact silvia.demarchis@unito and email@example.com by including an updated and detailed CV and a motivation letter.
Bonzano S, Crisci I, Podlesny-Drabiniok A, Rolando C, Krezel W, Studer M, De Marchis S. Neuron- Astroglia Cell Fate Decision in the Adult Mouse Hippocampal Neurogenic Niche Is Cell-Intrinsically Controlled by COUP-TFI In Vivo. Cell Rep. 2018 Jul 10;24(2):329-341. doi: 10.1016/j.celrep.2018.06.044.
Flore G, Di Ruberto G, Parisot J, Sannino S, Russo F, Illingworth EA, Studer M, De Leonibus E. Gradient COUP-TFI Expression Is Required for Functional Organization of the Hippocampal Septo-Temporal Longitudinal Axis. Cereb Cortex. 2017 Feb 1;27(2):1629-1643. doi: 10.1093/cercor/bhv336. PMID: 26813976.
Beckervordersandforth R. Mitochondrial Metabolism-Mediated Regulation of Adult Neurogenesis. Brain Plast. 2017 Nov 9;3(1):73-87. doi: 10.3233/BPL-170044.