RAUZI

Matteo RAUZI

Morphogenesis and mechanics of epithelial tissues

Main interests

  • Tissue mechanics
  • Bridging scales from cell to the embryo to understand morphogenesis during development
  • Live imaging, quantitative biology, mechanical and genetic manipulation

Scientific Questions

From cell mechanics to embryo morphogenesis

We are focused in understanding how cellular and sub-cellular properties are integrated at the embryo scale to give rise to emerging mechanisms necessary to drive coordinated tissue flows and tissue remodeling during development. The projects developed in the lab gather people from different backgrounds (biology, informatics, physics, and engineering) to generate an interdisciplinary and synergistic group in an international environment.

Our Strategy

Developmental biology is a field of great interest since it allows studying cells in a physiological relevant context. That is why scientists have been considering the embryo as an interesting “environment” in which to analyze and learn more about the biology and physics of cells. While much work has been done in dissecting cellular and subcellular properties, little is known of how nano and micro scale mechanisms are integrated at the embryo and how emerging properties arise to drive coordinated tissue flows and tissue remodeling responsable for morphogenesis and impacting on cell fate determination. The research we do aims to bridge scales from the subcellular to the embryo. This represents the ultimate understanding of how an embryo changes its shape during development.

The research we do in the lab aims to push further both the understanding of embryo development and the technology necessary to tackle such understanding. We use and develop cutting edge imaging techniques, laser manipulation, magnetic tweezers, optogenetic-based synthetic morphology, and image analysis with systematic BIG data processing. The study is done comparatively on wild type and mutated embryos. in silico modelling is implemented to delineate a formal physical framework that can theoretically reproduce morphogenetic processes and predict features of the system that are then back tested experimentally.

Research Aims

Tissue fold formation is a common morphological process taking place during morphogenesis. Such a process plays a key role in embryo development since it allows translocating cells in inner zones of the embryo where specific organs of the mature animal will then originate (process named gastrulation).

A model system that is particularly suited for studying folding is for example the Drosophila embryo for which many genetic tools are available and several manipulation tools can be applied to probe cell mechanics. In the early Drosophila embryo it has been shown that a tissue can fold via different mechanisms.

How can a tissue, during fold formation, change its curvature from convex to concave?

How are forces distributed in time at the surface and in the bulk of the embryo to drive morphogenesis?

Finally, how do tissue mechanics and morphogenesis impact on EMT, cell migration and cell fate determination?

Postdocs

DELORME Barthélemy - +33 492076948
POPKOVA Anna - +33 492076948

PreDocs

JOHN Alphy - +33 492076948

Engineers & Technicians

SCHORK FOUMSOU Soumaita - +33

 

Recent Publications

  1. Rauzi, M, Krzic, U, Saunders, TE, Krajnc, M, Ziherl, P, Hufnagel, L et al.. Embryo-scale tissue mechanics during Drosophila gastrulation movements. Nat Commun. 2015;6 :8677. doi: 10.1038/ncomms9677. PubMed PMID:26497898 PubMed Central PMC4846315.
  2. Collinet, C, Rauzi, M, Lenne, PF, Lecuit, T. Local and tissue-scale forces drive oriented junction growth during tissue extension. Nat. Cell Biol. 2015;17 (10):1247-58. doi: 10.1038/ncb3226. PubMed PMID:26389664 .
  3. Bajoghli, B, Kuri, P, Inoue, D, Aghaallaei, N, Hanelt, M, Thumberger, T et al.. Noninvasive In Toto Imaging of the Thymus Reveals Heterogeneous Migratory Behavior of Developing T Cells. J. Immunol. 2015;195 (5):2177-86. doi: 10.4049/jimmunol.1500361. PubMed PMID:26188059 .
  4. Rauzi, M, Hočevar Brezavšček, A, Ziherl, P, Leptin, M. Physical models of mesoderm invagination in Drosophila embryo. Biophys. J. 2013;105 (1):3-10. doi: 10.1016/j.bpj.2013.05.039. PubMed PMID:23823218 PubMed Central PMC3699736.
  5. Hočevar Brezavšček, A, Rauzi, M, Leptin, M, Ziherl, P. A model of epithelial invagination driven by collective mechanics of identical cells. Biophys. J. 2012;103 (5):1069-77. doi: 10.1016/j.bpj.2012.07.018. PubMed PMID:23009857 PubMed Central PMC3433605.
  6. Rauzi, M, Lenne, PF. Cortical forces in cell shape changes and tissue morphogenesis. Curr. Top. Dev. Biol. 2011;95 :93-144. doi: 10.1016/B978-0-12-385065-2.00004-9. PubMed PMID:21501750 .
  7. Rauzi, M, Lenne, PF, Lecuit, T. Planar polarized actomyosin contractile flows control epithelial junction remodelling. Nature. 2010;468 (7327):1110-4. doi: 10.1038/nature09566. PubMed PMID:21068726 .
  8. Bertet, C, Rauzi, M, Lecuit, T. Repression of Wasp by JAK/STAT signalling inhibits medial actomyosin network assembly and apical cell constriction in intercalating epithelial cells. Development. 2009;136 (24):4199-212. doi: 10.1242/dev.040402. PubMed PMID:19934015 .
  9. Rauzi, M, Lecuit, T. Closing in on mechanisms of tissue morphogenesis. Cell. 2009;137 (7):1183-5. doi: 10.1016/j.cell.2009.06.009. PubMed PMID:19563750 .
  10. Rauzi, M, Verant, P, Lecuit, T, Lenne, PF. Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis. Nat. Cell Biol. 2008;10 (12):1401-10. doi: 10.1038/ncb1798. PubMed PMID:18978783 .
  11. Cavey, M, Rauzi, M, Lenne, PF, Lecuit, T. A two-tiered mechanism for stabilization and immobilization of E-cadherin. Nature. 2008;453 (7196):751-6. doi: 10.1038/nature06953. PubMed PMID:18480755 .
Search PubMed

Technician in Drosophila fly genetics at the iBV (Institute of Biology Valrose) in Nice, FRANCE

The iBV is part of the Côte D’Azur University and is a leading international biology institute focused both in fundamental and medical oriented research.

The iBV is seeking a highly motivated technician willing to work on new challenging and exciting projects in the field of developmental biology. The work will be conducted in the new lab of the young group leader Dr. Matteo RAUZI focused in studying the morphogenesis and mechanics of epithelial tissues from cell to embryo (http://ibv.unice.fr/EN/equipe/rauzi.php). Matteo is seeking a candidate that is enthusiastic, hardworking, organized, efficient, responsive and willing to help setting up the new lab. Knowledge and skills in fly genetics and possibly confocal microscopy are requested. The position will initially be funded for 12 months with a possible extension. Candidates should provide a CV, a brief statement of their research interest and possibly one recommendation letter.

The iBV is within the beautiful campus Valrose that gatherers institutes of research in hard sciences. Valrose is located in the heart of the sunny city of Nice in the middle of the stunning Côte D’Azur.
Contact address: matteo.rauzi@unice.fr

Engineer/technician in image analysis and microscopy at the iBV (Institute of Biology Valrose) in Nice, FRANCE

The iBV is part of the Côte D’Azur University and is a leading international biology institute focused both in fundamental and medical oriented research.

The iBV is seeking a highly motivated engineer/technician willing to work on new challenging and exciting projects in the field of biophysics. The work will be conducted in the new lab of the young group leader Dr. Matteo RAUZI focused in studying the morphogenesis and mechanics of epithelial tissues from cell to embryo (http://ibv.unice.fr/EN/equipe/rauzi.php). Matteo is seeking a candidate that is enthusiastic, hardworking, organized, efficient, responsive and willing to help setting up the new lab. Knowledge and skills in microscopy, live imaging, image processing, quantitative analysis, programming (Matlab) are requested. The position will initially be funded for 12 months with a possible extension. Candidates should provide a CV, a brief statement of their research interest and possibly one recommendation letter.

The iBV is within the beautiful campus Valrose that gatherers institutes of research in hard sciences. Valrose is located in the heart of the sunny city of Nice in the middle of the stunning Côte D’Azur.

Contact address: matteo.rauzi@unice.fr

Postdoctoral position in Matteo RAUZI laboratory

Institute of Biology Valrose (iBV), Nice, France

The iBV is seeking a highly motivated young scientist willing to develop a new challenging and exciting project as a postdoc in the field of developmental biology. The work will be conducted in the new lab of the young group leader Matteo RAUZI focused in studying the morphogenesis and mechanics of epithelial tissues from cell to embryo by using cutting edge live imaging techniques, laser based manipulation, optogenetics, image analysis, BIG data processing, and in silico modelling.
(http://ibv.unice.fr/EN/equipe/rauzi.php)

Location
The group is part of the iBV, an international research center that brings together high-profile teams with complementary areas of expertise and with a common interest in translating basic research into knowledge for the clinic.
Scientific staff has access to state of the art facilities including imaging/microscopy and cytometry platforms, L2 and L3 laboratories, and histology and biochemistry facilities.
The iBV is located at the beautiful Valrose Campus in the heart of Nice in the middle of the stunning Côte D’Azur.
For more information, visit http://ibv.unice.fr

Qualification and experience
Matteo is seeking a candidate capable to conduct his/her scientific work with independent and free thinking and willing to participate in setting up the new lab. The Rauzi lab aims to gather people from different backgrounds (biology, informatics, physics, and engineering) to generate an interdisciplinary and synergistic group in an international environment within the iBV. Knowledge and skills in live imaging, image processing, quantitative analysis, basic programming (Matlab), fly genetics are much appreciated but not compulsory. Candidates should provide a brief statement of their research interest and at least one recommendation letter.

Applications
The position will initially be funded for 18 months and then eventually extended.
Interested applicants should send a C.V., a motivation letter and at least one reference letter.

matteo.rauzi@unice.fr
Tel : +33(0)492076352

Matteo RAUZI, PhD
Group Leader, institute of Biology Valrose (iBV)
Morphogenesis and Mechanics of Epithelial Tissues

iBV - Institut de Biologie Valrose

"Sciences Naturelles"

Université Nice Sophia Antipolis
Faculté des Sciences
Parc Valrose
06108 Nice cedex 2