Imaging Facility

PRISM

Imaging Facility

Presentation

The imaging facility PRISM (Platform of Resources in Imaging and Scientific Microscopy) has been created to develop and provide access to state-of-the-art microscopes and together with expertise in optical microscopy and image analysis.
Fully integrated in the iBV on Valrose campus, the platform coordinates the imaging needs and provides expertise for research groups using a wide variety of biological models including cultured cells, yeast (S. cerevisiae and C. albicans), worms (C. elegans), flies (D. melanogaster), zebrafish, and mice, to address a range of fundamental scientific questions using different approaches.

 

The Imaging facility is also accessible to external users. Within MICA - Microscopy and Imaging Côte d'Azur - , PRISM is part of a joint imaging facility for 6 institutes which has been labelled by the GIS IBiSA. Based on this partnership, these different facilities coordinate their development to provide the largest panel of advanced microscopy techniques. Hence, PRISM facility is open to a wide variety of academic and industrial partners in the scientific community.

PRISM facility is part of the national networks RTmfm and the GDR ImaBio. Thus the platform is in contact with other facilities to exchange knowledge and tools about state of the art microscopy technologies.

Wide field

  • Axiocolor : AxioPlan Zeiss / Color camera for coloration
  • Axiofluo : AxioPlan Zeiss / Black and White camera for fluorescence

 

Video microscope

  • Videobserveur : AxioObserver - Zeiss (2011) / EMCCD ANDOR iXON 897 camera and sCMOS ANDOR Neo camera / MetaMorph software
  • Timelaps: Axiovert 200M - Zeiss (2004) / sCMOS ANDOR Neo camera / MetaMorph software

 

Laser Scanning Confocal

  • Leica TCS SPE Microscope (2007)
  • Leica SP5 Microscope (2011): Hybrid detector / Galvanometric stage / resonant scanner
  • Zeiss LSM710 (2012): spectral detector
  • Zeiss LSM780 (2011) : sensitive spectral detector (GaAsp) / Biphoton laser (MaiTai) / NDD detector in fluorescence and transmission
  • Zeiss LSM880 (2018) : sensitive internal detector (GaAsp) / Fast AiryScan detector / Piezo stage

 

Spinning Disk

  • Spinning disk: Olympus/Andor/Yokogawa X1 (2010) : Dual cam module (EM-CCD) / Fast 3D (piezo stage)
  • SPIN-FRAP-TIRF: Nikon TiE/Yokogawa W1 (2016) : photomanipulation module (Ilas2) / sCMOS camera zyla 4.2+

 

Image analysis

  • Imaris for 3D analysis
  • Hygens for deconvolution

 

Engineer :

Simon Lachambre
Tel : +33 4 92 07 64 29
E-mail : slachambre@unice.fr

Development :

Sebastien Schaub
Tel : +33 4 92 07 64 28
E-mail : schaub@unice.fr

Scientific Coordinators :

Florence Besse
Tel : +33 4 92 07 64 34
E-mail : besse@unice.fr

Maximilian Furthauer
Tel : +33 4 92 07 64 40
E-mail : furthauer@unice.fr

iBV - Institut de Biologie Valrose

"Centre de Biochimie"

Université Nice Sophia Antipolis
Faculté des Sciences
Parc Valrose
06108 Nice cedex 2

iBV - Institut de Biologie Valrose

"Sciences Naturelles"

Université Nice Sophia Antipolis
Faculté des Sciences
Parc Valrose
06108 Nice cedex 2