Cytometry Facility

BV-CyAn

Flow Cytometry Platform

Presentation

The Cytometry Platform (acronym BV-CyAn) has been created in 2012 to provide access and develop this technology to the iBV research groups and is open to outside investigators. It offers a range of services centered on multiparametric cytometry and cell sorting.

BV-CyAn platform, by offering state-of-the-art flow cytometers and sorters, proposes to researchers, powerful ways to discriminate, analyse and sort/purify the distinct cell/organelle populations of interest to further study the genomic, proteomic and functional aspects of such purified populations.

Services

Provide access to cytometer users of the platform

teaching (users quickly become autonomous and independent in the use of analyzers), assistance (in the design of experiments, in the analysis, interpretation and presentation of results) and expertise

cell sorting
Provide technological development with new technical achievements

Analysor LSR Fortessa Sorp (BD)

* 5 lasers (488, 633, 561, 355, 405nm)
18 fluorescent signals
20000 evt/s

*A module HTS allow screening on 96wells plates

*A forward scatter PMT upgrade is also available for small particle detection (0.3-1µm)

Cell sorter  ARIA (BD):

4 lasers (488, 633, 561, 405nm)

13 fluorescent signals

2 and 4 way sorting

MESO QuickPlex SQ12:

MSD products are based on MULTI-ARRAY technology, which uses electrochemiluminescence detection.

* best sensitivity and dynamic range
* Single and multiplex assays
* Wide menu of commercially available kits and components for user-developed assays

https://www.mesoscale.com/

Technical Manager

Loubat Agnès
loubat@unice.fr
+33 4 92 07 68 71

Scientific Coordinators

Hueber Anne Odile
hueber@unice.fr

Schedl Andreas
schedl@unice.fr

 

iBV - Institut de Biologie Valrose

"Sciences Naturelles"

Université Nice Sophia Antipolis
Faculté des Sciences
Parc Valrose
06108 Nice cedex 2