Membrane trafficking and developmental signalling in animal development
- Delta/Notch signaling and neuro-epithelial polarity
- Developmental functions of the ESCRT machinery
- Extracellular vesicles in animal development
- Establishing Left/Right asymmetry: from cell polarity to organismal laterality
Cellular membranes are essential for the functional compartmentalization of the organism. Within cells, they enable the formation of specialized functional domains at the level of the plasma membrane or intracellular organelles. At the level of the organism, they provide interfaces that delimit the organism from the outside world and individual cells within tissues. Last not least, an increasing body of evidence suggests that membrane-bound extracellular vesicles play an important role in animal development and physiology. The overall aim of our research is to understand how the formation, function and dynamic remodeling of membrane-bound compartments governs the localization and activity of signaling molecules in animal development.
Our group has been using two complementary model systems, zebrafish and Drosophila to address two major questions:
First, how does endocytic membrane trafficking regulate developmental signalling?
Delta ligands and Notch receptors define a signaling pathway of major importance for development and disease. Notch receptor activation is dependent on Delta ligand endocytosis, but the actual mechanistic reason for this requirement is not fully understood. We established a novel assay to track endogenous signaling molecules in vivo and identified the asymmetric segregation of Delta-containing endosomes as a novel mechanism to promote directional Notch signaling in asymmetrically dividing neural precursor cells (Coumailleau et al 2009, Kressmann et al, 2015).
Second, how does a major membrane remodeling machinery control embryonic development?
The Endosomal Sorting Complex Required for Transport (ESCRT) is an evolutionarily conserved membrane-remodeling machinery that was initially discovered for its function in the endocytic regulation of growth factor signaling. Our work suggests that another important function of the ESCRT machinery is to promote the virus-like cell surface budding of extracellular vesicles that ensure the transport of developmental signaling molecules such as the major developmental morphogen Hedgehog (Matusek et al, 2014, Juan and Fürthauer, 2018).
Notch receptor activation has been proposed to rely on the apico-basal transport of endocytosed Delta ligand molecules. Through the development of novel live imaging assays we are now able to visualize this process in vivo, in the context of the development of the zebrafish neural tube. Using a combination of genetic and live imaging approaches, we are aiming to dissect the functional links between neuro-epithelial polarity and Delta/Notch signalling.
The Endosomal Sorting Complex Required for Transport (ESCRT) is well known for its essential role in the endocytic regulation of growth factor signaling. In contrast, how other ESCRT-dependent processes such as the secretion of extracellular vesicles contribute to animal development remains largely to be understood. A second major aim of our work is to characterize novel functions of this major membrane trafficking machinery in animal development.
In contrast to intracellular endosomes, Extracellular Vesicles (EVs) cannot use cytoskeleton-dependent molecular motors for their movement. We study how oriented motile cilia are used to create directional fluid flows in the extracellular space to drive EV transport. We have shown that Myosin1D controls motile cilia orientation in the zebrafish left-right organizer, identifying this actin-binding motor protein as a conserved regulator of animal left-right asymmetry (Juan et al, 2018).
- Saraswathy, VM, Kurup, AJ, Sharma, P, Polès, S, Poulain, M, Fürthauer, M et al.. The E3 ubiquitin ligase mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation. Elife. 2022;11 :. doi: 10.7554/eLife.71928. PubMed PMID:35142609 PubMed Central PMC8937233.
- Sharma, P, Saraswathy, VM, Xiang, L, Fürthauer, M. Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis. Sci Rep. 2019;9 (1):9958. doi: 10.1038/s41598-019-46067-1. PubMed PMID:31292468 PubMed Central PMC6620349.
- Matusek, T, Thérond, P, Fürthauer, M. Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc. Methods Mol Biol. 2019;1998 :31-47. doi: 10.1007/978-1-4939-9492-2_3. PubMed PMID:31250292 .
- Juan, T, Géminard, C, Coutelis, JB, Cerezo, D, Polès, S, Noselli, S et al.. Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry. Nat Commun. 2018;9 (1):1942. doi: 10.1038/s41467-018-04284-8. PubMed PMID:29769531 PubMed Central PMC5955935.
- Fürthauer, M. The ESCRT machinery: When function follows form. Semin Cell Dev Biol. 2018;74 :1-3. doi: 10.1016/j.semcdb.2017.11.003. PubMed PMID:29113869 .
- Juan, T, Fürthauer, M. Biogenesis and function of ESCRT-dependent extracellular vesicles. Semin Cell Dev Biol. 2018;74 :66-77. doi: 10.1016/j.semcdb.2017.08.022. PubMed PMID:28807885 .
- Mamińska, A, Bartosik, A, Banach-Orłowska, M, Pilecka, I, Jastrzębski, K, Zdżalik-Bielecka, D et al.. ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors. Sci Signal. 2016;9 (411):ra8. doi: 10.1126/scisignal.aad0848. PubMed PMID:26787452 .
- Juan, T, Fürthauer, M. [The ESCRT complex: from endosomal transport to the development of multicellular organisms]. Biol Aujourdhui. 2015;209 (1):111-24. doi: 10.1051/jbio/2015009. PubMed PMID:26115716 .
- Loubéry, S, Seum, C, Moraleda, A, Daeden, A, Fürthauer, M, Gonzalez-Gaitan, M et al.. Uninflatable and Notch Control the Targeting of Sara Endosomes during Asymmetric Division. Curr Biol. 2015;25 (6):817-818. doi: 10.1016/j.cub.2015.02.053. PubMed PMID:29665399 .
- Kressmann, S, Campos, C, Castanon, I, Fürthauer, M, González-Gaitán, M. Directional Notch trafficking in Sara endosomes during asymmetric cell division in the spinal cord. Nat Cell Biol. 2015;17 (3):333-9. doi: 10.1038/ncb3119. PubMed PMID:25706234 .
- Matusek, T, Wendler, F, Polès, S, Pizette, S, D'Angelo, G, Fürthauer, M et al.. The ESCRT machinery regulates the secretion and long-range activity of Hedgehog. Nature. 2014;516 (7529):99-103. doi: 10.1038/nature13847. PubMed PMID:25471885 .
- Loubéry, S, Seum, C, Moraleda, A, Daeden, A, Fürthauer, M, Gonzalez-Gaitan, M et al.. Uninflatable and Notch control the targeting of Sara endosomes during asymmetric division. Curr Biol. 2014;24 (18):2142-2148. doi: 10.1016/j.cub.2014.07.054. PubMed PMID:25155514 .
- Fürthauer, M, Smythe, E. Systems dynamics in endocytosis. Traffic. 2014;15 (3):338-46. doi: 10.1111/tra.12147. PubMed PMID:24405722 .
- Cavodeassi, F, Del Bene, F, Fürthauer, M, Grabher, C, Herzog, W, Lehtonen, S et al.. Report of the Second European Zebrafish Principal Investigator Meeting in Karlsruhe, Germany, March 21-24, 2012. Zebrafish. 2013;10 (1):119-23. doi: 10.1089/zeb.2012.0829. PubMed PMID:23530760 .
- Fürthauer, M, González-Gaitán, M. Endocytosis and mitosis: a two-way relationship. Cell Cycle. 2009;8 (20):3311-8. doi: 10.4161/cc.8.20.9700. PubMed PMID:19770584 .
- Fürthauer, M, González-Gaitán, M. Endocytosis, asymmetric cell division, stem cells and cancer: unus pro omnibus, omnes pro uno. Mol Oncol. 2009;3 (4):339-53. doi: 10.1016/j.molonc.2009.05.006. PubMed PMID:19581131 PubMed Central PMC5527947.
- Fürthauer, M, González-Gaitán, M. Tales of 1001 functions: the multiple roles of membrane trafficking in development. Traffic. 2009;10 (7):781-2. doi: 10.1111/j.1600-0854.2009.00931.x. PubMed PMID:19570190 .
- Fürthauer, M, González-Gaitán, M. Endocytic regulation of notch signalling during development. Traffic. 2009;10 (7):792-802. doi: 10.1111/j.1600-0854.2009.00914.x. PubMed PMID:19416471 .
- Dal-Pra, S, Fürthauer, M, Van-Celst, J, Thisse, B, Thisse, C. Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity. Dev Biol. 2006;298 (2):514-26. doi: 10.1016/j.ydbio.2006.07.002. PubMed PMID:16890217 .
- Poulain, M, Fürthauer, M, Thisse, B, Thisse, C, Lepage, T. Zebrafish endoderm formation is regulated by combinatorial Nodal, FGF and BMP signalling. Development. 2006;133 (11):2189-200. doi: 10.1242/dev.02387. PubMed PMID:16672336 .
Postdoc/PhD position at the Institut de Biologie Valrose
Functional analysis of Myosin 1C/D in Zebrafish Left/Right asymmetry
The team of Maximilian Fürthauer at the Institut de Biologie Valrose in Nice (http://ibv.unice.fr/EN/equipe/furthauer.php) is looking for a highly motivated doctoral or postdoctoral research fellow to study the role of MyosinI proteins in establishing zebrafish Left/Right asymmetry.
While well conserved molecular pathways govern antero-posterior and dorso-ventral patterning in different phyla, highly divergent mechanisms have been proposed to be responsible for the specification of Left/Right asymmetry in different organisms. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, cilia-independent mechanisms have been suggested to involve localized ion flows or directional cellular rearrangements dependent on cytoskeletal polarity. This raises the question whether a unifying mechanism controlling Left/Right asymmetry still remains to be identified? An attractive hypothesis is that cytoskeletal chirality may provide a template for organismal laterality.
In Drosophila, the actin-binding molecular motor protein Myosin 1D (Myo1D) acts as an essential determinant of Left/Right asymmetry. Myo1D is itself negatively regulated by Myosin 1C (Myo1C), establishing the Myosin 1C/D system as a central regulator of Left/Right asymmetry in flies. The objective of this project at the interface between cellular and developmental biology is to dissect the contribution of the Myosin 1C/D system to the establishment of zebrafish Left/Right asymmetry. This work will be performed in the context of a highly active ANR-funded collaboration with the lab of Stéphane Noselli, who has pioneered the study of Myo1D in Drosophila Left/Right asymmetry.
Our project aims to determine the function of Myosin 1C/D, its spatial and temporal requirements, identify its partners and targets. The interaction of the Myosin 1C/D system with known Left/Right pathways (nodal, cilia, actin cytoskeleton) will be studied, leading to an integrated model of the establishment of Left/Right. The candidate will greatly benefit from different tools that the Fürthauer lab has already established to study different aspects Left/Right asymmetry and manipulate Zebrafish Myosin 1C/D function.
We particularly encourage applications by candidates with expertise in developmental or cellular biology that are motivated to apply quantitative approaches to the image-based analysis of biological processes.
Candidates should send their CV, a description of their scientific achievements and the contacts of two referees to Maximilian Fürthauer (firstname.lastname@example.org).
2011 - HFSP Career Development Award
2010 - ATIP/Avenir Junior Group Leeder Programme
2005 - Long Term Postdoctoral Fellowship, HFSP
2004 - Postdoctoral fellowship, EMBO
Thomas Juan, PhD student of Maximilian Fürthauer’s team at iBV, winner of the Bettencourt Prize for Young Researchers 2019
iBV - Institut de Biologie Valrose
Université Nice Sophia Antipolis
Faculté des Sciences
06108 Nice cedex 2