Molecular programs controlling development and tissue homeostasis
- Signals directing self-renewal and differentiation of renal stem cells
- Transcriptional control of glomerular podocyte differentiation
- Adrenal tissue homeostasis and its links to cancer
- Sex-specific differences of non-reproductive organs
Organ development is a highly complex process that requires the orchestrated action of transcription factors and signalling pathways. Once an organ has formed it needs to be maintained to ensure proper functioning throughout the entire life. To achieve this feat, many organs employ stem cells that can be activated to renew the damaged organ. Past research has demonstrated that development and tissue maintenance are highly interrelated and molecular programs driving development and differentiation are also triggered when stem cells become activated to replace damaged or lost cells. Focusing on the kidney and adrenal glands, our research program aims at understanding the transcriptional control underlying tissue development, define stem/progenitor cells in the adult organism and determine the signalling pathways that are involved in their maintenance and activation.
Kidneys are central cardiovascular organs that ensure blood filtration and - together with the adrenals - control blood pressure, pH and electrolyte concentration. Kidney diseases are on the rise and it is expected that as many as 1 in 10 people will suffer from renal disease at some stage during their life. Moreover, defective blood pressure control leads to hypertension, the foremost cause of death worldwide. Given the high incidence of these diseases, new therapeutic approaches are urgently required. A vision for the future is to employ regenerative medicine that would permit the growth of new organs from a patient’s own cells. To achieve this goal an in depth knowledge of how kidneys and adrenals form and how they are maintainted is required. In our lab, we are concentrating on three main topics: 1) We address how proliferation of renal stem cells is regulated and identify molecular networks directing their differentiation into functional nephrons. 2) We study podocytes, a key component of the renal filtration barrier, and address how differentiation and maintenance of this unique cell type is achieved. 3) We identify the molecular pathways required for adrenal cortex specification and maintenance. To achieve our goals we employ state of the art genetic (inducible knockouts, CRISPR/Cas9, lineage tracing, organoids) and molecular techniques (ChIP-Seq, scRNA-Seq) and analyze gene function in vitro and in vivo.
Nephron progenitors represent a developmental stem cell population that fuels nephron formation and determines the total number of nephrons per kidney. Progenitors need to constantly decide between quiescence, proliferation and differentiation, which is achieved through signalling pathways that induce specific transcriptional responses. We are focussing on the transcriptional regulators of the SOXC class, identify their role in these processes and determine the molecular networks they regulate.
Podocytes are highly specialized cells that form unique cell extensions (footprocesses) required for glomerular filtration. Podocyte damage is one of the main mechanisms leading to end stage renal failure thus underlining the importance of this cell type. We are interested in identifying the transcriptional program orchestrating podocyte differentiation and maintenance and understand how the unique structure and function of this cell type is acquired and maintained.
The adult adrenal cortex undergoes constant tissue renewal. To achieve this feat cells proliferate at the outer cortex and migrate in a centripetal fashion changing their differentiation along their differentiation path only to undergo apoptosis at the cortico-medullary boundary. We are studying the molecular pathways that ensure maintenance of organ size and function, and address how disruption of tissue dynamics can lead to adrenal diseases, including cancer.
VIDAL Valérie - +33 489150735
Engineers & Technicians
- Lyraki, R, Schedl, A. The Sexually Dimorphic Adrenal Cortex: Implications for Adrenal Disease. Int J Mol Sci. 2021;22 (9):. doi: 10.3390/ijms22094889. PubMed PMID:34063067 PubMed Central PMC8124132.
- Lyraki, R, Schedl, A. Adrenal cortex renewal in health and disease. Nat Rev Endocrinol. 2021;17 (7):421-434. doi: 10.1038/s41574-021-00491-4. PubMed PMID:34011989 .
- Oikonomakos, I, Weerasinghe Arachchige, LC, Schedl, A. Developmental mechanisms of adrenal cortex formation and their links with adult progenitor populations. Mol Cell Endocrinol. 2021;524 :111172. doi: 10.1016/j.mce.2021.111172. PubMed PMID:33484742 .
- Russell, JP, Lim, X, Santambrogio, A, Yianni, V, Kemkem, Y, Wang, B et al.. Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells. Elife. 2021;10 :. doi: 10.7554/eLife.59142. PubMed PMID:33399538 PubMed Central PMC7803373.
- Comai, GE, Tesařová, M, Dupé, V, Rhinn, M, Vallecillo-García, P, da Silva, F et al.. Local retinoic acid signaling directs emergence of the extraocular muscle functional unit. PLoS Biol. 2020;18 (11):e3000902. doi: 10.1371/journal.pbio.3000902. PubMed PMID:33201874 PubMed Central PMC7707851.
- Werdermann, M, Berger, I, Scriba, LD, Santambrogio, A, Schlinkert, P, Brendel, H et al.. Insulin and obesity transform hypothalamic-pituitary-adrenal axis stemness and function in a hyperactive state. Mol Metab. 2021;43 :101112. doi: 10.1016/j.molmet.2020.101112. PubMed PMID:33157254 PubMed Central PMC7691554.
- Bornstein, SR, Malyukov, M, Heller, C, Ziegler, CG, Ruiz-Babot, G, Schedl, A et al.. New Horizons: Novel Adrenal Regenerative Therapies. J Clin Endocrinol Metab. 2020;105 (9):. doi: 10.1210/clinem/dgaa438. PubMed PMID:32629476 PubMed Central PMC7398608.
- Chassot, AA, Le Rolle, M, Jolivet, G, Stevant, I, Guigonis, JM, Da Silva, F et al.. Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary. Sci Adv. 2020;6 (21):eaaz1261. doi: 10.1126/sciadv.aaz1261. PubMed PMID:32494737 PubMed Central PMC7244317.
- Steenblock, C, Todorov, V, Kanczkowski, W, Eisenhofer, G, Schedl, A, Wong, ML et al.. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the neuroendocrine stress axis. Mol Psychiatry. 2020;25 (8):1611-1617. doi: 10.1038/s41380-020-0758-9. PubMed PMID:32382135 PubMed Central PMC7204611.
- Vidal, VP, Jian-Motamedi, F, Rekima, S, Gregoire, EP, Szenker-Ravi, E, Leushacke, M et al.. R-spondin signalling is essential for the maintenance and differentiation of mouse nephron progenitors. Elife. 2020;9 :. doi: 10.7554/eLife.53895. PubMed PMID:32324134 PubMed Central PMC7228766.
- Scriba, LD, Bornstein, SR, Santambrogio, A, Mueller, G, Huebner, A, Hauer, J et al.. Cancer Stem Cells in Pheochromocytoma and Paraganglioma. Front Endocrinol (Lausanne). 2020;11 :79. doi: 10.3389/fendo.2020.00079. PubMed PMID:32158431 PubMed Central PMC7051940.
- Kozlov, VM, Schedl, A. Duplex kidney formation: developmental mechanisms and genetic predisposition. F1000Res. 2020;9 :. doi: 10.12688/f1000research.19826.1. PubMed PMID:32030122 PubMed Central PMC6945105.
- Wang, Y, Wu, H, Fontanet, P, Codeluppi, S, Akkuratova, N, Petitpré, C et al.. A cell fitness selection model for neuronal survival during development. Nat Commun. 2019;10 (1):4137. doi: 10.1038/s41467-019-12119-3. PubMed PMID:31515492 PubMed Central PMC6742664.
- Grabek, A, Dolfi, B, Klein, B, Jian-Motamedi, F, Chaboissier, MC, Schedl, A et al.. The Adult Adrenal Cortex Undergoes Rapid Tissue Renewal in a Sex-Specific Manner. Cell Stem Cell. 2019;25 (2):290-296.e2. doi: 10.1016/j.stem.2019.04.012. PubMed PMID:31104943 .
- Massa, F, Schedl, A. Awakening the Bowman: inhibition of CXCL12 signaling activates parietal epithelial cells. Kidney Int. 2018;94 (6):1042-1044. doi: 10.1016/j.kint.2018.09.011. PubMed PMID:30466560 .
- Schedl, A. The author replies. Kidney Int. 2018;94 (4):827. doi: 10.1016/j.kint.2018.06.015. PubMed PMID:30243318 .
- Da Silva, F, Massa, F, Motamedi, FJ, Vidal, V, Rocha, AS, Gregoire, EP et al.. Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4. Dev Biol. 2018;441 (1):42-51. doi: 10.1016/j.ydbio.2018.05.024. PubMed PMID:29859889 PubMed Central PMC6365680.
- Neirijnck, Y, Reginensi, A, Renkema, KY, Massa, F, Kozlov, VM, Dhib, H et al.. Sox11 gene disruption causes congenital anomalies of the kidney and urinary tract (CAKUT). Kidney Int. 2018;93 (5):1142-1153. doi: 10.1016/j.kint.2017.11.026. PubMed PMID:29459093 .
- Comai, G, Boutet, A, Tanneberger, K, Massa, F, Rocha, AS, Charlet, A et al.. Genetic and Molecular Insights Into Genotype-Phenotype Relationships in Osteopathia Striata With Cranial Sclerosis (OSCS) Through the Analysis of Novel Mouse Wtx Mutant Alleles. J Bone Miner Res. 2018;33 (5):875-887. doi: 10.1002/jbmr.3387. PubMed PMID:29329488 .
- Da Silva, F, Rocha, AS, Motamedi, FJ, Massa, F, Basboga, C, Morrison, H et al.. Coronary Artery Formation Is Driven by Localized Expression of R-spondin3. Cell Rep. 2017;20 (8):1745-1754. doi: 10.1016/j.celrep.2017.08.004. PubMed PMID:28834739 .
2018 - Equipe labellisée – Ligue contre le Cancer
2012 - Equipe labellisée - ARC
2009 - Award of the French kidney foundation (Prix de la Fondation du Rein)
2009 - Equipe labellisée - FRM
2007 - Member of the Faculty of 1000 Medecine
2006 - Equipe labellisée - FRM
2003 - Avenir - Inserm
2002 - Philippe Leverhulme Prize (UK)
2001 - Young Investigator Programme - EMBO
iBV - Institut de Biologie Valrose
"Centre de Biochimie"
Université Nice Sophia Antipolis
Faculté des Sciences
06108 Nice cedex 2