Sci Rep. 2020 Feb 6;10(1):1942. doi: 10.1038/s41598-019-54349-x.
Gustavo de Medeiros1,2, Dimitri Kromm1,3, Balint Balazs1,4, Nils Norlin1, Stefan Günther1, Emiliano Izquierdo1, Paolo Ronchi1, Shinya Komoto1,5, Uros Krzic1, Yannick Schwab1, Francesca Peri1,6, Stefano de Renzis1, Maria Leptin1, Matteo Rauzi7,8, Lars Hufnagel9
1 European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
2 Friedrich Miescher Institute for Biomedical Research, Maulbeerstr. 66, CH-4058, Basel, Switzerland.
3 Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany.
4 Luxendo GmbH, Kurfürsten-Anlage 58, 69115, Heidelberg, Germany.
5 Imaging Section, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa, 904-0495, Japan.
6 Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
7 European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany. firstname.lastname@example.org.
8 Université Côte d’Azur, CNRS, Inserm, iBV, Nice, France. email@example.com.
9 European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany. firstname.lastname@example.org.
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.