1 Post-Doc Position - 3 years - Starting October 2018

POST-DOC POSITION IN CELL AND DEVELOPMENTAL BIOLOGY.

INSTITUTE OF BIOLOGY-VALROSE, NICE, FRANCE

Position available (starting oct./dec. 2018) in Pascal Thérond laboratory to functionally characterize Hedgehog secretion and spreading in Drosophila. Hedgehog proteins are key signaling mediators that govern a wide range of processes during embryonic development and adult tissue homeostasis from Drosophila to human. Hedgehogs are the only metazoan proteins known to possess a covalently-linked cholesterol moiety. Our laboratory is interested in how Hedgehog proteins traffic in the producing tissue and exert their function in the receiving tissue.
We have shown that the Endosomal Sorting Complex Required for Transport (ESCRT) promotes Hedgehog proteins loading on exo-vesicles to exert their effect at long distances. The post-doctoral project aims to gain further insight into the trafficking, vesicular secretion and the extracellular spread of Hedgehog proteins through tissues using cell biology and genetic technics. Moreover, in vivo imaging and single molecule tracking (in collaboration with computational science lab) has been developped on our tissue models and will be further used to investigate the dynamics of Hedgehog release and spreading.

Interested candidates should have strong knowledge of, and experience in fly genetics, cell biology and optic microscopy (confocal/spinning disc). The position is funded for 3 years in duration. Candidates must have a Ph.D. degree, and can be nationals of any country.

Selected references: Ayers et al., Dev. Cell 2010 vol18, 605–620; Briscoe and Thérond, Nat Rev Mol Cell Biol. Vol. 14, 2013; Matusek et al., Nature 2014 Dec 4;516(7529): 99-103; D’Angelo et al., Dev. Cell 2015 Feb. 9 ; 32, 290-303.

Candidates should send a Curriculum Vitae and a list of three referees to:
Dr. Pascal Therond, CNRS-UMR 6543, Université de Nice-Valrose, 06108 Nice Cedex 2, France.
Phone: (33) 4 92076446. Email: therond@unice.fr

CDD AI - 18 months - Starting October 2018

CDD Assistant Ingénieur, 18 mois

Laboratoire

iBV, Institut de Biologie Valrose, CNRS UMR7277, Inserm U1091, UNS

Equipe - Localisation du poste

BIPOA, Bio-Ingénierie et Physiopathologie Ostéo-Articulaire
(http://ibv.unice.fr/research-team/scimeca/)
Faculté de Médecine, 28 av de Valombrose

Poste à pourvoir

A partir de la mi-septembre 2018

Projet

Dans un partenariat académiques/industriels, le projet financé par la Banque Publique d’Investissement a pour objectif le développement d’une nouvelle génération de ciments phosphocalciques élastiques et résistants pour le traitement des fractures vertébrales.

Programme de travail et compétences requises

Le programme de travail repose sur l’étude des interactions entre les ciments phosphocalciques développés et les cellules osseuses (ostéoblastes, ostéoclastes), grâce à la mise en oeuvre des techniques suivantes :

• Préparation et mise en forme des ciments pour les tests in vitro ;
• Culture cellulaire et mesures de la viabilité/prolifération ;
• Transcriptomique (RT-qPCR) et protéomique (ELISA multiplex)
• Immunohistochimie

La personne sera aussi impliquée dans l’analyse d’implants en site osseux réalisés par nos collaborateurs responsables de l’expérimentation in vivo, grâce à une technique de microscopie quantitative (SHG, Second Harmonic Generation)
Sont attendus : rigueur et réactivité dans la conduite des expériences et dans la mise en forme des résultats ; aptitudes à interagir au sein d’un réseau impliquant 3 laboratoires académiques et 2 industriels

Contact

Jean-Claude SCIMECA
scimeca@unice.fr

1 PostDoc - 3 years - Start October 2018

POST-DOC POSITION AVAILABLE:
Regulation of neuronal RNA granules and function in long-term memory

A three-year post-doctoral position is available in the group of Florence Besse
(http://ibv.unice.fr/research-team/besse/) at the Institute of Biology Valrose (iBV-UMR7277, Nice, France).

Project description

Long-term molecular and structural changes occurring at synapses in response to neuronal activation provide the mechanistic bases for the establishment and retention of memories, and are altered in both developmental and age-related memory disorders. Such changes rely on local experience-induced translation of quiescent mRNAs packaged together with regulatory proteins into neuronal RNA granules transported and stored at synapses. How RNA granules are remodeled in response to neuronal activity to relieve translation repression of mRNAs is unclear. Furthermore, the importance of such a remodeling in the establishment of long-term memories remains to be demonstrated in vivo.
The successful candidate will address these questions in Drosophila, by dissecting the cellular and molecular mechanisms underlying the remodeling of conserved neuronal RNP granules in response to activity. She/he will also participate in characterizing how such a remodeling impacts on the establishment of long-term memory traces assessed via learning and memory behavior assays (in collaboration with K. Keleman’s lab).

Qualifications and Experience

Candidates should have a PhD in cell biology, molecular biology, genetics, or neurobiology.
Experience with fly genetics, behavior assays and/or transcriptomics will be considered advantageous.
English is the working language in the lab and in the Institute.

Funding

The position is funded for 3 years, and the starting date is October 2018

Applications

Interested candidates are encouraged to send a CV, a description of research interests and accomplishments, and names and contact information of 2 referees to Florence Besse: besse@unice.fr.

Selected references:

- De Graeve F. and Besse F. (2018). Biol.chem doi: 10.1515/hsz-2018-0141
- Medioni C., Ephrussi A. and Besse F. (2015). Nat. Protoc. doi: 10.1038/nprot.2015.034.
- Marchetti G. et al., (2014). J. Neurosci. doi: 10.1523/JNEUROSCI.3285-13.2014.
- Medioni C. et al., (2014). Cur Biol. doi: 10.1016/j.cub.2014.02.038.

1 PhD Position - 3 years - Starting September 2018

PhD Fellowship in Developmental Biology

Maternal factors regulating Nodal expression in the sea urchin embryo:
a mechanistic and evolutionary approach

A three-year PhD position supported by the French Foundation for Medical Research (FRM) is available starting September 1st 2018 to work in the group Gene regulatory networks, axis specification and morphogenesis of the sea urchin embryo at the Institute of Biology Valrose. The area of research will concern the analysis of Dorsal-ventral patterning in the sea urchin embryo through the analysis of the function and evolutionary conservation of several maternal factors that play critical roles in the regulation of nodal expression and/or Nodal activity. (see Lapraz F, Haillot E, and Lepage,T – 2015 - A deuterostome origin of the Spemann organiser suggested by Nodal and ADMPs functions in Echinoderms; Nature communications and Haillot E, Molina MD, Lapraz F, and Lepage,T (2015) The Maternal Maverick/GDF15-like TGF-β Ligand Panda Directs Dorsal-Ventral Axis Formation by Restricting Nodal Expression in the Sea Urchin Embryo. - 2015 - PLoS Biology.

Our laboratory has recently identified several maternal factors that play crucial roles in the regulation of nodal expression including a maternal TGF beta called Panda, a BMP type I receptor called Alk1/2, a maternal transcription factor of the Ets family called Yan as well a as novel secreted protein of unknown function. Although these factors are each crucially required to spatially restrict nodal expression, their mechanism of action is not understood. Furthermore, it is not known if these genes are required to restrict nodal expression only in the Mediterranean sea urchin Paracentrotus lividus or if their function is conserved in other species.
The project will specifically address these questions by elucidating how these factors work and determining if their function is conserved in different species.

Candidates should have obtained recently a Master degree. Both national and international candidates are encouraged to apply. Interested candidates should send a Curriculum Vitae, a summary of research interests and goals and contact information for two or three referees to:

Dr Thierry Lepage : tlepage@unice.fr
Research team webpage

Institut de Biologie Valrose
CNRS UMR7277 – Inserm U1091
Université Nice Sophia Antipolis 06108 Cedex 2, France

1 CDD AI - 12 months - Open January 2018

 1 poste d’assistant ingénieur/ingénieur

en oncoimmunologie fondamentale et translationnelle des cancers des VADS

Le Centre Antoine Lacassagne (Nice) et les équipes du Dr Ellen Van Obberghen (iBV, Nice) et du Dr Fabienne Anjuère (IPMC, Valbonne) ont mis en place un consortium afin de potentialiser des travaux collaboratifs en oncoimmunologie fondamentale et translationnelle des cancers des voies aéro-digestives supérieures (VADS). Ce consortium s’intéresse à la compréhension des interactions entre le stroma tumoral et les cellules immunitaires dans les cancers des VADS afin d’identifier de nouveaux marqueurs pronostiques et de nouvelles approches d’intervention thérapeutique.

Dans ce cadre, le consortium recrute un personnel technique, niveau ingénieur ou assistant-ingénieur, en CDD pour une période de 12 mois renouvelables. Le candidat sera chargé de la gestion de biopsies humaines de cancers des VADS dans le cadre de recherches fondamentales et d’essais cliniques d’immunothérapie, de l‘analyse de la signature immunitaire et stromale de ces biopsies humaines par des approches complémentaires d’imagerie en fond clair et de fluorescence et de cytométrie de flux. Ces analyses multiparamétriques requièrent de plus des compétences en informatique et en gestion et archivage de données.

Profil recherché

  •  Expérience en histologie (coupes de biopsies incluses en paraffine et cryocongelées, coloration de lames, marquages en immunohistochimie et en immunofluorescence)
  •  Expérience en cytométrie de flux multiparamétrique et connaissances en immunologie également souhaitées.
  •  Capacité à développer et rédiger des protocoles standardisés
  •  Qualités d’organisation, rigueur et esprit d’équipe indispensables
  •  Disponibilité et flexibilité d’horaires liée au travail sur biopsies humaines fraiches à processer extemporanément

Lieu d’activité: Le site principal de l’activité sera basé à l’IBV, Faculté des Science, Nice, et requerra des missions régulières sur les sites de recherché du Centre Antoine Lacassagne (Centre de Ressources Biologiques), Nice et de l’IPMC, Valbonne.

Conditions de recrutement

  •  Contrat initial d’une durée de 12 mois renouvelable
  •  Niveau de rémunération dépendante de l’expérience professionnelle, selon la grille de rémunération du Centre Antoine Lacassagne
  •  Poste à pourvoir à partir de janvier 2018

Contact: Les candidatures (CV, lettre de motivation et noms de deux référents) sont à adresser par courriel à Christine Lovera (christine.lovera@nice.unicancer.fr), Directrice, Direction de la Recherche Clinique et de l'Innovation, Centre Antoine Lacassagne.