Author: Jean-Claude SCIMECA

Interdisciplinary Doctoral Project
Interdisciplinary Doctoral Project
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Interdisciplinary Doctoral project in the RAUZI lab (University Côte d’Azur, IBV, Nice) and in the ETIENNE lab (Univ Grenoble Alpes, LIPHY, Grenoble), at the interface between computational physics and biology

Studying the mechanisms and mechanics driving tissue folding

Mophogenesis builds living shapes. A key morphogenetic transformation that shapes tissues and organs is epithelial invagination: a tissue bends and it is eventually internalized transforming the physiological topology of the system. The invagination of epithelial tissues is a vital transformation during embryo development since it is pivotal during embryo gastrulation and neurulation. While much is known of the mechanisms and mechanics driving epithelial flattening (first phase) and bending (second phase), how a tissue is eventually internalized (third phase) is still poorly understood. To tackle this, we propose to use the Drosophila embryo that provides the most advanced genetic tools and study the process of mesoderm internalization. On the computational physics side, we will develop a formal physical framework that can theoretically reproduce morphogenetic processes and predict features of the system that are then back tested experimentally. More specifically, we will design a mechanical model based on active viscoelastic shells and use numerical simulations based on existing tools (e.g., Surface Evolver in 3D) to calculate shell deformations. On the biology side, we will implement multi-view light sheet microscopy coupled to optogenetics and plasma-based laser ablation and image data analysis to characterize and synthetically modulate tissue shape changes to test numerical predictions. The student will be trained on these multiple approaches and techniques to develop an interdisciplinary project focused on uncovering the fundamental principles governing epithelial folding. This knowledge could be used in the future to synthetically build and shape functional organs.The project will be developed in both the Rauzi lab (http://ibv.unice.fr/research-team/rauzi/) and the Etienne lab (http://www-liphy.univ-grenoble-alpes.fr/pagesperso/etienne).

We are seeking a highly motivated and talented candidate to develop this interdisciplinary PhD project. Send a CV, a motivation letter, master scores/ranking and reference letters to matteo.rauzi@univ-cotedazur.fr and jocelyn.etienne@univ-grenoble-alpes.fr

Technician Position in Spinal Cord Regeneration Research
Technician Position in Spinal Cord Regeneration Research
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The Cigliola laboratory invites applications for a Research Technician position at the Institute of Biology Valrose in Nice, France. Our lab uses zebrafish and mouse models to study Innate Mechanisms of Spinal Cord Regeneration. We are looking for highly motivated and ambitious candidates with interest for regenerative biology and neuroscience. Enthusiasm for working with animals is essential. Specialized training will be provided. The candidate will have the opportunity to figure as co-author on published manuscripts.

The Institute of Biology Valrose is a research Center of Excellence at the University of Côte d’Azur and offers a vibrant research environment with national and international scientists and collaborations (iBV). Nice is located on the French Riviera and offers amazing opportunities for outdoor recreation.

Technical duties include:

  • Zebrafish breeding, husbandry, and surgeries; molecular biology; generation of mutant or transgenic animals by microinjection.
  • Contribution to research studies, experiments, data collection and assays.
  • Maintenance of general lab supplies; ordering and preparation of reagents and solutions; assistancewith maintaining equipment and lab reagent inventories.
  • Review of laboratory methods manuals.
  • Implementation and supervision of laboratory safety procedures.Required qualifications:Bachelor’s degree in biology or a related field and at least 2-years previous experience in a research lab. The qualified candidate should have strong troubleshooting skills, should be motivated to learn new techniques, organized, and able to work effectively in a team environment. Previous experience with zebrafish is preferred (but not required). Candidates should be fluent in English, as it is the working language in the laboratory.

Please send your application in a single PDF, including (1) a cover letter that includes a description of research interests and experience, (2) CV and (3) contact information of 2 or more references to Valentina CIGLIOLA. Review of applications will begin immediately, and the position will remain open until filled.

PhD Student and Postdoc Positions in Spinal Cord Regeneration Research
PhD Student and Postdoc Positions in Spinal Cord Regeneration Research
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The Cigliola laboratory invites applications for PhD Student and Postdoc positions at the Institute of Biology Valrose in Nice, France. Our lab uses zebrafish and mouse models to study Mechanisms of Spinal Cord Regeneration.

While in adult mammals (including humans) spinal cord injury causes lifelong paralysis, zebrafish possess an astounding capacity to regenerate and recover function after a paralyzing injury. We are interested in the following questions: What are the factors and mechanisms allowing innate spinal cord regeneration to occur? How are these mechanisms regulated? Can regeneration in adult mammals be enhanced by re-establishment of innate pro-regenerative programs?

We are looking for curiosity-driven, motivated and ambitious candidates with interest for regenerative biology and neuroscience. We strongly value team spirit, a positive work environment and collaborations. Enthusiasm for working with zebrafish and/or mice is essential. Candidates should be fluent in English, as it is the working language in the laboratory.

The Institute of Biology Valrose is a research Center of Excellence at the University of Côte d’Azur and offers a vibrant research environment with national and international scientists and collaborations (iBV). Nice is located on the French Riviera and offers amazing opportunities for outdoor recreation.

Please send your application in a single PDF, including (1) a cover letter that includes a description of research interests and experience, (2) CV and (3) contact information of 2 or more references to Valentina CIGLIOLA. Review of applications will begin immediately, and the position will remain open until filled.