RNA granules in neuronal plasticity and disease

Trends Neurosci. 2023 May 16;S0166-2236(23)00104-2. DOI: 10.1016/j.tins.2023.04.004

RNA granules in neuronal plasticity and disease

Karl E Bauer¹, Bruna R de Queiroz², Michael A Kiebler³, Florence Besse⁴

Affiliation

¹Biomedical Center (BMC), Department of Anatomy and Cell Biology, Medical Faculty, Ludwig-Maximilians-University, Planegg-Martinsried, Germany.
²Université Côte d’Azur, CNRS, Inserm, Institut de Biologie Valrose, Nice, France.
³Biomedical Center (BMC), Department of Anatomy and Cell Biology, Medical Faculty, Ludwig-Maximilians-University, Planegg-Martinsried, Germany.
⁴Université Côte d’Azur, CNRS, Inserm, Institut de Biologie Valrose, Nice, France.

Abstract

RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus and the cytoplasm where they assemble with target RNAs to form multi-molecular complexes. Here, we describe a protocol to selectively identify RNAs associated with RBPs of interest in the cytoplasmic and nuclear compartments of adult Drosophila brain cells. Cytoplasmic and nuclear fractions are differentially collected and used for immunoprecipitation-based purification of GFP-tagged RBPs. This protocol can be applied to samples expressing ectopic or endogenous tagged RBPs.

DOI: 10.1016/j.tins.2023.04.004