Maternal eating behavior is a major synchronizer of fetal and postnatal peripheral clocks in mice.

Cell Mol Life Sci. 2018 Nov;75(21):3991-4005. doi: 10.1007/s00018-018-2845-5. Epub 2018 May 26.

Canaple L¹, Gréchez-Cassiau A², Delaunay F², Dkhissi-Benyahya O³, Samarut J⁴.

Author information

1. Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon; CNRS UMR 5242, Institut de Génomique Fonctionnelle de Lyon, 46 allée d’Italie, 69364, Lyon, France. laurence.canaple@ens-lyon.fr.
2. Université Côte d’Azur, CNRS UMR7277, INSERM U1091, Institut de Biologie Valrose, Bâtiment de Sciences Naturelles, 28 Avenue Valrose, 06108, Nice Cedex 2, France.
3. Université de Lyon, Université Claude Bernard Lyon 1, Inserm, Stem Cell and Brain Research Institute U1208, 69500, Bron, France.
4.Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon; CNRS UMR 5242, Institut de Génomique Fonctionnelle de Lyon, 46 allée d’Italie, 69364, Lyon, France. Jacques.Samarut@ens-lyon.fr.

Abstract

Most living organisms show circadian rhythms in physiology and behavior. These oscillations are generated by endogenous circadian clocks, present in virtually all cells where they control key biological processes. To study peripheral clocks in vivo, we developed an original model, the Rev-Luc mouse to follow noninvasively and longitudinally Rev-Luc oscillations in peripheral clocks using in vivo bioluminescence imaging. We found in vitro and in vivo a robust diurnal rhythm of Rev-Luc, mainly in liver, intestine, kidney and adipose tissues. We further confirmed in vivo that Rev-Luc peripheral tissues are food-entrainable oscillators, not affected by age or sex. These data strongly support the relevance of the Rev-Luc model for circadian studies, especially to investigate in vivo the establishment and the entrainment of the rhythm throughout ontogenesis. We then showed that Rev-Luc expression develops dynamically and gradually, both in amplitude and in phase, during fetal and postnatal development. We also demonstrate for the first time that the immature peripheral circadian system of offspring in utero is mainly entrained by maternal cues from feeding regimen. The prenatal entrainment will also differentially determine the Rev-Luc expression in pups before weaning underlining the importance of the maternal chrononutrition on the circadian system entrainment of the offspring.

PMID: 29804258
DOI:10.1007/s00018-018-2845-5