Expression patterns of sterol transporters NPC1 and NPC2 in ...


Dani V, Priouzeau F, Mertz M, Mondin M, Pagnotta S, Lacas-Gervais S, Davy SK, Sabourault C. in Cell Microbiol. 2017 Oct,19(10). doi: 10.1111/cmi.12753.


Expression patterns of sterol transporters NPC1 and NPC2 in the cnidarian-dinoflagellate symbiosis.

The symbiotic interaction between cnidarians (e.g., corals and sea anemones) and photosynthetic dinoflagellates of the genus Symbiodinium is triggered by both host-symbiont recognition processes and metabolic exchange between the 2 partners. The molecular communication is crucial for homeostatic regulation of the symbiosis, both under normal conditions and during stresses that further lead to symbiosis collapse. It is therefore important to identify and fully characterise the key players of this intimate interaction at the symbiotic interface. In this study, we determined the cellular and subcellular localization and expression of the sterol-trafficking Niemann-Pick type C proteins (NPC1 and NPC2) in the symbiotic sea anemones Anemonia viridis and Aiptasia sp. We first established that NPC1 is localised within vesicles in host tissues and to the symbiosome membranes in several anthozoan species. We demonstrated that the canonical NPC2-a protein is mainly expressed in the epidermis, whereas the NPC2-d protein is closely associated with symbiosome membranes. Furthermore, we showed that the expression of the NPC2-d protein is correlated with symbiont presence in healthy symbiotic specimens. As npc2-d is a cnidarian-specific duplicated gene, we hypothesised that it probably arose from a subfunctionalisation process that might result in a gain of function and symbiosis adaptation in anthozoans. Niemann-Pick type C proteins may be key players in a functional symbiosis and be useful tools to study host-symbiont interactions in the anthozoan-dinoflagellate association.

   Ccile Sabourault - 28/11/2017 Plus d'informations sur :
Liste publications  



  Contacts :
      - Webmasters
      - Directeur
iBV - Institut de Biologie Valrose
CNRS UMR7277, Inserm U1091, UNS Université Nice Sophia Antipolis
Parc Valrose
06108 Nice cedex 2 France
L'institut :
  - Présentation
  - Organigramme
  - Nos Soutiens
  - Imagerie (PRISM)
  - Cytométrie
  - Biologie Moléculaire
  - Proteïne & Purification
  - Histopathologie
  - Offres de postes
  - Accès
Groupes de recherche :
- Amri Ez-Zoubir
- Arkowitz Robert
- Besse Florence
- Braendle Christian
- Chaboissier Marie-Christine
- Collombat Patrick
- Dani Christian
- Delaunay Franck
- Fürthauer Maximilian
- Gouze Elvire
- Hubstenberger Arnaud
- Hueber Anne-Odile
- Lamonerie Thomas
- Léopold Pierre
- Lepage Thierry
- Equipe Morpheme
- Noselli Stéphane
- Rassoulzadegan Minoo
- Rauzi Matteo
- Sandoz Guillaume
- Schedl Andreas
- Scimeca Jean-Claude
- Soriani Olivier
- Studer Michele
- Pascal Thérond
- Ellen Van Obberghen-Schilling
- Virolle Thierry
- Wagner Kay Dietrich
Actualités :
  - Publications
  - Actualités
  - Offres de postes
Agenda :
  - Conférences
  - HDR
  - Thèses
  - Liste globale
  - Congrès
Nous Contacter :
  - Direction
  - Groupes de recherche
  - Services techniques
  - Annuaire général