Sex determination in mice

Genetics of sex determination and fertility in mammals.
In mammals, the pivotal decision to develop as male or female begins with the differentiation of the bipotential gonad allowing the development of two highly specialized organs: the testis or the ovary. In addition the gonad is the siege of germ cells differentiation that insures the propagation of species. It is now clear that the fate of the gonad and germ cells are closely linked, however, cellular and molecular mechanisms leading to somatic or germ cell differentiation are still not fully understood. Our aim is to contribute to unravelling these mechanisms involved in normal and pathologic development of testes and ovaries.

Male development : testicular differentiation.
Sexual differentiation is determined by the paternal transmission of the SRY gene located on the Y chromosome. The expression of Sry promotes testicular differentiation by up-regulation of the transcription factor Sox9. However, using mice models, we have shown that testicular differentiation can occur in the absence of Sry and Sox9 expression. The identification of these new genes is of great importance to understand how sex determination occurs in other vertebrate in which Sry and Sox9 have been shown not to be the sex determining factors. New potential sexual determinants are under investigation in our lab.


Female development : ovarian differentiation.
R-spondins genes (RSPO) are activators of WNT/beta-catenin signaling pathway and regulate various developmental processes and proliferation of transiently amplifying stem cells. Mutations of RSPO1 are responsible of female-to-male sex reversal and/or ovarian carcinomas predisposition indicating that Rspo1 controls ovarian differentiation. In addition, Rspo1/beta-catenin signaling pathway is also required for proliferation of germ cells and their commitment to meiosis. The different functions of the Rspo1/beta-catenin signalling pathway in sex determination and fertility are deciphered in our lab using mouse models.


Last publications

Constitutive WNT/CTNNB1 activation triggers spermatogonial stem cell proliferation and germ cell depletion. - 2017 - Developmental biology - 426 P17-27 - Chassot AA, Le Rolle M, Jourden M, Taketo MM, Ghyselinck NB, and Chaboissier,MC

Retinoic acid signaling is dispensable for somatic development and function in the mammalian ovary. - 2017 - Developmental biology - 424 P208-220 - Minkina A, Lindeman RE, Gearhart MD, Chassot AA, Chaboissier MC, Ghyselinck NB, Bardwell VJ, and Zarkower,D

R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways. - 2017 - Cell reports - 18 P2320-2330 - Lacour F, Vezin E, Bentzinger CF, Sincennes MC, Giordani L, Ferry A, Mitchell R, Patel K, Rudnicki MA, Chaboissier MC, Chassot AA, and Le Grand,F

Amplification of R-spondin1 signaling induces granulosa cell fate defects and cancers in mouse adult ovary. - 2017 - Oncogene - 36 P208-218 - De Cian MC, Pauper E, Bandiera R, Vidal VP, Sacco S, Gregoire EP, Chassot AA, Panzolini C, Wilhelm D, Pailhoux E, Youssef SA, de Bruin A, Teerds K, Schedl A, Gillot I, and Chaboissier,MC

Involvement of FOXL2 and RSPO1 in Ovarian Determination, Development, and Maintenance in Mammals. - 2016 - Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation - 10 P167-184 - Pannetier M, Chassot AA, Chaboissier MC, and Pailhoux,E

Steroidogenic organ development and homeostasis: A WT1-centric view. - 2015 - Molecular and cellular endocrinology - Bandiera R, Sacco S, Vidal VP, Chaboissier MC, and Schedl,A

Ovarian development and disease: The known and the unexpected. - 2015 - Seminars in cell & developmental biology - 45 P59-67 - Biason-Lauber A, and Chaboissier,MC

R-spondin1 deficiency enhances β-Cell neogenesis in a murine model of diabetes. - 2014 - Pancreas - 43 P93-102 - Chahal JK, Wong VS, Chaboissier MC, and Brubaker,PL

Marker genes identify three somatic cell types in the fetal mouse ovary. - 2014 - Developmental biology - 394 P242-52 - Rastetter RH, Bernard P, Palmer JS, Chassot AA, Chen H, Western PS, Ramsay RG, Chaboissier MC, and Wilhelm,D

R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation. - 2014 - Reproduction (Cambridge, England) - 148 PR97-110 - Chassot AA, Gillot I, and Chaboissier,MC

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Chaboissier Marie-Christine
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Members Team

    April 2015 - M.C. Chaboissier Team
   1 CDD Technicien AI 1 an   Closed



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